BAC Bacteriostatic Water

BAC Bacteriostatic Water

Bacteriostatic Water represents a sterile, pyrogen-free aqueous preparation containing benzyl alcohol at a concentration of 0.9% (v/v), which serves as the preservative agent. This solution has been developed for laboratory applications, functioning as either a carrier or diluting agent in scenarios requiring multiple withdrawals while ensuring sterile conditions are maintained.

Within research settings, Bacteriostatic Water sees frequent use in creating experimental dilutions, solubilizing peptides or proteins, and rehydrating reagents. The low-level bacteriostatic component permits convenient repeated access while safeguarding both assay dependability and sample quality.

BAC Bacteriostatic Water Overview

This preparation finds application in laboratory workflows demanding regular access to a sterile solvent under rigorous aseptic protocols. It proves especially useful in experimental contexts where preventing contamination and achieving uniformity are paramount. Standard uses encompass dissolving peptides and small-molecule substances, diluting reagents for subcellular or tissue culture experiments, and formulating calibration standards for analytical equipment.

Apart from its general function as a sterile carrier, the formulation is engineered to deliver reproducibility and chemical constancy across diverse biochemical and cellular investigations. It enables precision in studies involving metabolic assays, peptide development and preservation, and the protection of delicate reagents, thereby underpinning superior research results in analytical and molecular biology applications.

BAC Bacteriostatic Water Structure

Chemical Makeup

Bacteriostatic Water consists of sterile water (H₂O) enhanced with benzyl alcohol at 0.9% (v/v). Given that this solution constitutes a straightforward preserved aqueous carrier rather than a distinct compound, assigning a single definitive molecular formula beyond the solvent system would be inappropriate. Analytical confirmation for this production lot encompasses mass spectrometric and chromatographic validation of water purity and preservative concentration.

• Observed Mass (MS): 711.9 Da
• Purity (HPLC): 99.42%
• Batch Number: 2025007
• Primary Retention Time: 3.48 min
• Instrument: LCMS-7800 Series (Calibrated)
• Analytical Note: Primary peak validates water/preservative makeup; trace secondary peak area 0.58%

BAC Bacteriostatic Water Research

Solvent for Peptide and Protein Reconstitution

This preparation is purposefully engineered for rehydrating freeze-dried peptides and proteins within aseptic laboratory settings. It permits researchers to formulate sterile solutions while reducing contamination hazards, even when accessing the same vial repeatedly. The consistent composition guarantees compatibility with an extensive array of biomolecules, fostering reproducible outcomes in peptide synthesis, protein assays, and analytical procedures.

Diluent for Reagent Preparation

It operates as a dependable and practical diluting agent for creating reagent solutions demanding uniform sterility and chemical steadiness. The formulation suits the production of reagent stocks, calibration fluids for analytical instruments, or working solutions employed in biochemical, molecular, and cell-based assays. Its physicochemical characteristics help maintain reagent integrity throughout storage and repeated handling.

Vehicle for Experimental Administration

In controlled research contexts, this sterile solution can function as a carrier for non-clinical investigational preparations, enabling accurate dosing and reproducibility. Its multi-use design permits repeated withdrawals from a single sterile container without sacrificing purity, rendering it particularly beneficial in preclinical and pharmacological investigations.

This product is designated exclusively for laboratory and research applications conducted by qualified professionals. It is unsuitable for human or veterinary use or any clinical administration.

Article Author

This literature review was assembled, revised, and structured by Dr. John R. Vane, D.Sc., FRS. Dr. Vane was an eminent British pharmacologist and Nobel Prize recipient acknowledged for his revolutionary findings in prostaglandin and vascular pharmacology. His trailblazing work on prostacyclin (PGI₂) and prostaglandin E (PGE) clarified fundamental mechanisms governing vascular tone, platelet activity, and inflammation regulation. Dr. Vane's meticulousness in experimental pharmacology and guidance in assay methodology contributed to shaping modern standards for sterile solution formulation and pharmaceutical-grade water systems utilized in research and manufacturing.

Scientific Journal Author

The scientific heritage of Dr. John R. Vane created essential frameworks for pharmacological testing, biochemical validation, and the creation of reproducible solvent systems such as bacteriostatic water. Collaborating with distinguished colleagues—Sir Salvador Moncada, R.J. Gryglewski, and S. Bunting—Dr. Vane enhanced comprehension of how solvent composition influences chemical stability, assay reliability, and compound delivery in laboratory environments.

This recognition celebrates Dr. Vane's considerable impact on the advancement of experimental formulation principles guiding contemporary laboratory solvent and reagent design. It is offered for scientific attribution purposes only and does not suggest endorsement, sponsorship, or affiliation. BAC Research holds no professional connection with Dr. Vane or any of his associates.

Reference Citations

1. Bacteriostatic Water for Injection, USP - DailyMed Label
2. Study.com. Bacteriostatic Water: Definition and Uses
3. Tydes Research Blog. Bacteriostatic Water Role in Peptide Research
4. Elements Arms. Bacteriostatic Water vs Sterile Water: Understanding the Differences
5. PubChem Compound Summary for Benzyl Alcohol
6. Centers for Disease Control and Prevention. Laboratory Water Quality Control
7. U.S. Pharmacopeial Convention. Water for Pharmaceutical Purposes
8. ClinicalTrials.gov Identifier: NCT05060296. Investigation of adjuvant solvents in peptide preparation